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Theobromine is the main alkaloid found in both chocolate and cocoa. Did you know Cocoa powder can differ in the amount of theobromine, from 2% theobromine, up to greater levels around 10%. Also, Cocoa butter only contains small amounts of theobromine. There are usually greater concentrations in dark than in milk chocolate. Theobromine can also be found in trace amounts in the kola nut , the guarana berry, yerba mate (Ilex paraguariensis), and the tea plant. Another fun fact is that 28 grams (1 oz) of milk chocolate contains approximately 60 milligrams (1 grain) of theobromine, while the same amount of dark chocolate contains about 200 milligrams (3 grains). Cocoa beans naturally contain approximately 1% theobromine.

Plant species and components with substantial amounts of theobromine are:[16]